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 | Acesso ao texto completo restrito à biblioteca da Embrapa Soja. Para informações adicionais entre em contato com valeria.cardoso@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Agrobiologia; Embrapa Soja. |
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Data corrente: |
10/03/2006 |
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Data da última atualização: |
04/10/2007 |
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Autoria: |
ALBERTON, O.; KASCHUK, G.; HUNGRIA, M. |
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Título: |
Sampling effects on the assessment of genetic diversity of rhizobia associated with soybean and common bean. |
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Ano de publicação: |
2006 |
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Fonte/Imprenta: |
Soil Biology and Biochemistry, Oxford, v. 38, p. 1298-1307, 2006. |
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Idioma: |
Inglês |
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Conteúdo: |
Biological nitrogen fixation plays a key role in agriculture sustainability, and assessment of rhizobial diversity contributes to worldwide knowledge of biodiversity of soil microorganisms, to the usefulness of rhizobial collections and to the establishment of long-term strategies aimed at increasing contributions of legume-fixed N to agriculture. Although in recent decades the use of molecular techniques has contributed greatly to enhancing knowledge of rhizobial diversity, concerns remain over simple issues such as the effects of sampling on estimates of diversity. In this study, rhizobia were isolated from nodules of plants grown under field conditions, in pots containing soil, or in Leonard jars receiving a 10-2 or a 10-4 serially-diluted soil inoculum, using one exotic (soybean, Glycine max) and one indigenous (common bean, Phaseolus vulgaris) legume species. The experiments were performed using an oxisol with a high population (105 cells g-1 soil) of both soybean rhizobia, composed of naturalized strains introduced in inoculants and of indigenous common-bean rhizobia. BOX-PCR was used to evaluate strain diversity, while RFLP-PCR of the ITS (internally transcribed spacer) region with five restriction enzymes aimed at discriminating rhizobial species. In both analyses the genetic diversity of common-bean rhizobia was greater than that of soybean. For the common bean, diversity was greatly enhanced at the 10-4 dilution, while for the soybean dilution decreased diversity. Qualitative differences were also observed, as the DNA profiles differed for each treatment in both host plants. Differences obtained can be attributed to dissimilarity in the history of the introduction of both the host plant and the rhizobia (exotic vs. indigenous), to host-plant specificity, rhizobial competitiveness, and population structure, including ease with which some types are released from microcolonies in soil. Therefore, sampling method should be considered both in the interpretation and comparison of the results obtained in different studies, and in the setting of the goals of any study, e.g. selection of competitive strains, or collection of a larger spectrum of rhizobia. Furthermore, effects of sampling should be investigated for each symbiosis. MenosBiological nitrogen fixation plays a key role in agriculture sustainability, and assessment of rhizobial diversity contributes to worldwide knowledge of biodiversity of soil microorganisms, to the usefulness of rhizobial collections and to the establishment of long-term strategies aimed at increasing contributions of legume-fixed N to agriculture. Although in recent decades the use of molecular techniques has contributed greatly to enhancing knowledge of rhizobial diversity, concerns remain over simple issues such as the effects of sampling on estimates of diversity. In this study, rhizobia were isolated from nodules of plants grown under field conditions, in pots containing soil, or in Leonard jars receiving a 10-2 or a 10-4 serially-diluted soil inoculum, using one exotic (soybean, Glycine max) and one indigenous (common bean, Phaseolus vulgaris) legume species. The experiments were performed using an oxisol with a high population (105 cells g-1 soil) of both soybean rhizobia, composed of naturalized strains introduced in inoculants and of indigenous common-bean rhizobia. BOX-PCR was used to evaluate strain diversity, while RFLP-PCR of the ITS (internally transcribed spacer) region with five restriction enzymes aimed at discriminating rhizobial species. In both analyses the genetic diversity of common-bean rhizobia was greater than that of soybean. For the common bean, diversity was greatly enhanced at the 10-4 dilution, while for the soybean dilution decreased diversity. ... Mostrar Tudo |
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Palavras-Chave: |
Diversidade genética; Rizóbio. |
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Thesagro: |
Rhizobium; Soja. |
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Thesaurus Nal: |
soybeans. |
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Categoria do assunto: |
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Marc: |
LEADER 02857naa a2200205 a 4500
001 1629207
005 2007-10-04
008 2006 bl --- 0-- u #d
100 1 $aALBERTON, O.
245 $aSampling effects on the assessment of genetic diversity of rhizobia associated with soybean and common bean.
260 $c2006
520 $aBiological nitrogen fixation plays a key role in agriculture sustainability, and assessment of rhizobial diversity contributes to worldwide knowledge of biodiversity of soil microorganisms, to the usefulness of rhizobial collections and to the establishment of long-term strategies aimed at increasing contributions of legume-fixed N to agriculture. Although in recent decades the use of molecular techniques has contributed greatly to enhancing knowledge of rhizobial diversity, concerns remain over simple issues such as the effects of sampling on estimates of diversity. In this study, rhizobia were isolated from nodules of plants grown under field conditions, in pots containing soil, or in Leonard jars receiving a 10-2 or a 10-4 serially-diluted soil inoculum, using one exotic (soybean, Glycine max) and one indigenous (common bean, Phaseolus vulgaris) legume species. The experiments were performed using an oxisol with a high population (105 cells g-1 soil) of both soybean rhizobia, composed of naturalized strains introduced in inoculants and of indigenous common-bean rhizobia. BOX-PCR was used to evaluate strain diversity, while RFLP-PCR of the ITS (internally transcribed spacer) region with five restriction enzymes aimed at discriminating rhizobial species. In both analyses the genetic diversity of common-bean rhizobia was greater than that of soybean. For the common bean, diversity was greatly enhanced at the 10-4 dilution, while for the soybean dilution decreased diversity. Qualitative differences were also observed, as the DNA profiles differed for each treatment in both host plants. Differences obtained can be attributed to dissimilarity in the history of the introduction of both the host plant and the rhizobia (exotic vs. indigenous), to host-plant specificity, rhizobial competitiveness, and population structure, including ease with which some types are released from microcolonies in soil. Therefore, sampling method should be considered both in the interpretation and comparison of the results obtained in different studies, and in the setting of the goals of any study, e.g. selection of competitive strains, or collection of a larger spectrum of rhizobia. Furthermore, effects of sampling should be investigated for each symbiosis.
650 $asoybeans
650 $aRhizobium
650 $aSoja
653 $aDiversidade genética
653 $aRizóbio
700 1 $aKASCHUK, G.
700 1 $aHUNGRIA, M.
773 $tSoil Biology and Biochemistry, Oxford$gv. 38, p. 1298-1307, 2006.
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Registro original: |
Embrapa Agrobiologia (CNPAB) |
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| Registros recuperados : 26 | |
| 1. |  | KASCHUK, G.; ALBERTON, O.; HUNGRIA, M. Quantificando os efeitos de diferentes usos agrícolas na biomassa microbiana do solo nos biomas brasileiros. In: REUNIÃO BRASILEIRA DE FERTILIDADE DO SOLO E NUTRIÇÃO DE PLANTAS, 29.; REUNIÃO BRASILEIRA SOBRE MICORRIZAS, 13.; SIMPÓSIO BRASILEIRO DE MICROBIOLOGIA DO SOLO, 11.; REUNIÃO BRASILEIRA DE BIOLOGIA DO SOLO, 8., 2010, Guarapari. Fontes de nutrientes e produção agrícola: modelando o futuro: anais. Viçosa: SBCS, 2010. 4 p. Trab. 437. 1 CD-ROM. FERTBIO 2010.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| 4. | | KASCHUK, G.; ALBERTON, O.; HUNGRIA, M. Três décadas de estudos sobre biomassa microbiana nos ecossistemas brasileiros: lições aprendidas sobre qualidade do solo e indicadores de sustentabilidade. In: REUNIÃO BRASILEIRA DE FERTILIDADE DO SOLO E NUTRIÇÃO DE PLANTAS, 29.; REUNIÃO BRASILEIRA SOBRE MICORRIZAS, 13.; SIMPÓSIO BRASILEIRO DE MICROBIOLOGIA DO SOLO, 11.; REUNIÃO BRASILEIRA DE BIOLOGIA DO SOLO, 8., 2010, Guarapari. Fontes de nutrientes e produção agrícola: modelando o futuro: anais. Viçosa: SBCS, 2010. 4 p. Trab. 1501. 1 CD-ROM. FERTBIO 2010.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| 6. | | LOUREIRO, M. de F.; KASCHUK, G.; ALBERTON, O.; HUNGRIA, M. Soybean [Glycine max (L.) Merrill] rhizobial diversity in Brazilian oxisols under various soil, cropping, and inoculation managements. Biology and Fertility of Soils, Berlin, v. 43, n. 6. p. 665-674, Aug. 2007.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Internacional - A |
| Biblioteca(s): Embrapa Soja. |
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| 9. | | BROWN, G. G.; BRANDÃO JUNIOR, O.; SARIDAKIS, G. P.; BENITO, N. P.; ALBERTON, O.; TORRES, E. Efeito de diferentes preparos do solo na macrofauna edáfica de um latossolo vermelho distroférrico em Londrina, PR. In: WORLD CONGRESS ON CONSERVATION AGRICULTURE = CONGRESSO MUNDIAL SOBRE AGRICULTURA CONSERVACIONISTA, 2., 2003, Foz do Iguaçu. Producing in harmony with nature: [proceedings ...] = Produzindo em harmonia com a natureza: [anais ...]. Ponta Grossa: Federação Brasileira de Plantio Direto na Palha, 2003. v. 2. p. 426-429. II World Congress on Conservation Agriculture, Iguassu Falls, 2003. Extended summary / posters.| Biblioteca(s): Embrapa Soja. |
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| 10. | | LOUREIRO, M. de F.; KASCHUK, G.; ALBERTON, O.; HUNGRIA, M.; CORREA, F. Diferenças qualitativas na microbiota do solo relacionadas ao manejo do solo e das culturas. In: REUNIÃO BRASILEIRA DE FERTILIDADE DO SOLO E NUTRIÇÃO DE PLANTAS, 25.; REUNIÃO BRASILEIRA SOBRE MICORRIZAS, 9.; SIMPÓSIO BRASILEIRO DE MICROBIOLOGIA DO SOLO, 7.; REUNIÃO BRASILEIRA DE BIOLOGIA DO SOLO, 4., 2002, Rio de Janeiro. Fertbio 2002: agricultura: bases ecológicas para o desenvolvimento social e econômico sustentado. Rio de Janeiro, 2002. p. 172. Guia do congressista e em CD-ROM.| Biblioteca(s): Embrapa Soja. |
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| 11. | | BROWN, G.; HUNGRIA, M.; TORRES, E.; OLIVEIRA, L.; BRANDÃO JUNIOR, O.; ALBERTON, O. A faúna do solo no sistema de semeadura direta: comunidade e função no sistema edáfico. In: HOFFMANN-CAMPO, C .B.; SARAIVA, O. F. (Org.). Resultados de pesquisa da Embrapa Soja - 2001: microbiologia de solos. Londrina: Embrapa Soja, 2002. p. 11-13. (Embrapa Soja. Documentos, 197).| Biblioteca(s): Embrapa Soja. |
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| 12. | | KASCHUK, G.; LEFFELAAR, P. A.; GILLER, K. E.; ALBERTON, O.; HUNGRIA, M.; KUYPER, T. W. Responses of legumes to rhizobia and arbuscular mycorrhizal fungi: a meta-analysis of potencial photosynthate limitation of symbioses. Soil Biology and Biochemistry, Elmsford, v. 41, n. 1, p. 125-127, jan. 2010.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Soja. |
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| 13. | | BROWN, G. G.; ALBERTON, O.; BRANDÃO JÚNIOR, O.; SARIDAKIS, G. P.; TORRES, E. Scarab beetle-grub holes in various tillage and crop management systems at Embrapa Soybean, Londrina, Brazil. In: INTERNATIONAL TECHNICAL WORKSHOP ON BIOLOGICAL MANAGEMENT OF SOIL ECOSYSTEMS FOR SUSTAINABLE AGRICULTURE, 2002, Londrina. Program, abstracts and related documents. Londrina: Embrapa Soybean: FAO, 2002. p.91. (Embrapa Soja. Documentos, 182). Organizado por George G. Brown, Mariangela Hungria, Lenita Jacob Oliveira, Sally Bunning, Adriana Montañez.| Biblioteca(s): Embrapa Soja. |
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| 14. | | BROWN, G. G.; BRANDÃO JUNIOR, O.; ALBERTON, O.; SARIDAKIS, G. P.; BENITO, N. P.; TORRES, E. Soil macroinvertebrate populations in various land management systems in Paraná State, Brazil. In: INTERNATIONAL COLLOQUIUM ON SOIL ZOOLOGY AND ECOLOGY, 14., 2004. Mont Saint Aignan. Abstracts. Mont Saint Aignan: Université de Rouen, 2004. p. 240.| Biblioteca(s): Embrapa Soja. |
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| 15. | | GOMZ, S. A.; GAZZONI, D. L.; ALBERTON, O. C.; RUMIATTO, M.; GOMES, V.; SOUZA, D. S. de; STAUT, R. E. Efeito de métodos de aplicação na eficiência de Baculovirus anticarsia sobre a lagarta da soja. In: CONGRESSO BRASILEIRO DE ENTOMOLOGIA, 9., 1984, Londrina. Resumos... Londrina: SEB, [1984?]. p. 150.| Biblioteca(s): Embrapa Soja. |
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| 16. | | BROWN, G. G.; OLIVEIRA, L. J.; NORTON, D.; ALBERTON, O.; BRANDÃO JUNIOR, O.; SARIDAKIS, G. P.; TORRES, E. Quantifying scarab beetle-grub holes and their volume as affected by different tillage and crop management systems. In: INTERNATIONAL SOIL TILLAGE RESEARCH ORGANISATION CONFERENCE, 16., 2003, Brisbane. Soil management for sustainability. Brisbane: Grains Research & Development Corporation: The University of Queensland: Australian Centre for International Agricultural Research, 2003. p. 213-218. 1 CD-ROM.| Biblioteca(s): Embrapa Soja. |
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| 17. | | BROWN, G. G.; BRANDÃO JÚNIOR, O.; ALBERTON, O.; HUNGRIA, M.; SILVA, S. H. da; TORRES, E.; OLIVEIRA, L. J. Soil management and soil microfauna communities at Embrapa Soybean, Londrina, Brazil. In: INTERNATIONAL TECHNICAL WORKSHOP ON BIOLOGICAL MANAGEMENT OF SOIL ECOSYSTEMS FOR SUSTAINABLE AGRICULTURE, 2002, Londrina. Program, abstracts and related documents. Londrina: Embrapa Soybean: FAO, 2002. p. 84-85. (Embrapa Soja. Documentos, 182). Organizado por George G. Brown, Mariangela Hungria, Lenita Jacob Oliveira, Sally Bunning, Adriana Montañez.| Biblioteca(s): Embrapa Soja. |
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| 18. | | BRANDÃO JUNIOR, O.; SARIDAKIS, G. P.; FERREIRA, M. C.; SARIDAKIS, H. O.; ESPÍNDOLA, C. R.; HUNGRIA. M.; ALBERTON, O.; TORRES, E. Avaliação da população e biomassa microbianas em diferentes sistemas de preparo do solo. In: CONGRESSO BRASILEIRO DE MICROBIOLOGIA, 21., 2001, Foz do Iguaçu-PR. Resumos... Foz do Iguaçu: Sociedade Brasileira de Microbiologia, 2001. Trab. MS-076.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| 19. | | GOMEZ, S. A.; GAZZONI, D. L.; ALBERTON, O. C.; GOMES, V.; VALENTE, J. B.; RUMIATTO, M.; SOUZA, D. S. de; STAUT, R. E. Efeito de Baculovirus anticarsia sobre a lagarta da soja em aplicação aérea e terrestre, nas condições de Mato Grosso do Sul. Dourados: EMBRAPA-UEPAE Dourados, 1984. 14 p. (EMBRAPA-UEPAE Dourados. Pesquisa em andamento, 18).| Biblioteca(s): Embrapa Agropecuária Oeste. |
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| 20. | | BROWN, G. G.; BRANDÃO JÚNIOR, O.; ALBERTON, O.; HUNGRIA, M.; SILVA, S. H. da; ALBINO, U.; TORRES, E.; OLIVEIRA, L. J. O manejo do solo e as comunidades da macrofauna edáfica de um latossolo roxo em Londrina, PR. In: CONGRESSO BRASILEIRO DE SOJA, 2.; MERCOSOJA 2002, 2002, Foz do Iguaçu. Perspectivas do agronegócio da soja: resumos. Londrina: Embrapa Soja, 2002. p. 131. (Embrapa Soja. Documentos, 181). Organizado por Odilon Ferreira Saraiva, Clara Beatriz Hoffmann-Campo.| Biblioteca(s): Embrapa Soja. |
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| Registros recuperados : 26 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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